goat polyclonal antibodies against epha2 (R&D Systems)
Structured Review

Goat Polyclonal Antibodies Against Epha2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat polyclonal antibodies against epha2/product/R&D Systems
Average 93 stars, based on 18 article reviews
Images
1) Product Images from "Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells"
Article Title: Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells
Journal: Biomedicines
doi: 10.3390/biomedicines10123234
Figure Legend Snippet: EphA2, ephrin-A1, EphB4, and ephrin-B1 localization in LSECs and Kupffer cells. Immunofluorescence micrographs showing EphA2, ephrin-A1, EphB4, and ephrin-B1 immunoreactivity (red) in CD146-positive LSECs (green) and F4/80-positive Kupffer cells (green) in the mouse liver. Cryostat sections were stained with the indicated antibodies and DAPI (blue). Green fluorescence images were merged with red, and/or blue fluorescence images in the same field. EphA2, ephrin-A1, EphB4, and ephrin-B1 immunoreactivity was localized in CD146-positive LSECs and weakly localized in F4/80-positive Kupffer cells (arrows). M, marginal region of the hepatic lobules; Pc, pericentral region of the hepatic lobules.
Techniques Used: Immunofluorescence, Staining, Fluorescence
Figure Legend Snippet: Expression of Ephs and ephrins in the mouse liver, liver Mø propagated using mixed culture, and LSECs. ( A ) The protein expression and tyrosine phosphorylation (PTyr) of EphA2 and EphB4 were detected using Western blotting in the mouse liver. EphA2 and EphB4 in the liver are highly and weakly tyrosine-phosphorylated, respectively. IP, immunoprecipitation. ( B , C ) RT-PCR analysis of all Ephs and ephrins in liver Mø propagated using mixed culture ( B ) and primary LSECs ( C ). Liver Mø express Epha2 , Epha4 , Efna1 , Efna4 , Efna5, Ephb3 , Ephb4 , Ephb6 , Efnb1 , Efnb2 , and Efnb3 whereas LSECs express Epha2 , Efna1 , Ephb4 , Efnb1 , Efnb2 , and Efnb3 .
Techniques Used: Expressing, Phospho-proteomics, Western Blot, Immunoprecipitation, Reverse Transcription Polymerase Chain Reaction